P16 C.ELEGANS

INTRODUCTION 

On the 15th of February we went to the PRBB, biomedical research park in Barcelona. There we did an activity that consisted in classifying some bugs (some worms called C. Elegans) according to the characteristics that they had (physical and genetic).

There were 4 small boxes:

- Wild C. elegans.
- GFP + RFP (fluorescence proteins)
- Daf 18 mutated (mutated gene, they had cut it so that C. Elegan was shorter).
- Daf 18 silenced (turn off a gene).

We divided into four groups (Microscopy, image, genetics and behaviour). One of our students went through the groups analysing what we were doing.

My group had the tasks of microscopy.

OBJECTIVES 

The aim of microscopy was to classify the C. Elegans from knowing that in the laboratories there are more hermaphrodites, so that they can reproduce better. Hermaphrodites have a pointed tail and the males have a round tail. 

PROCEDURE 

We take some microscopes and one magnifying glass to see the different boxes of c. elegans and we keep watching them and to check how many were males and hermaphrodites. We took notes in a paper. 

RESULTS 
These are the common results of all groups.

	TYPE (RESULT)	MICROSCOPY	IMAGE	GENETICS	BEHAVIOUR
A	DAF 18 mutated (FLUORESCENCE)	8 males 100 herma	I X F X	More shorter	More to the salt. (THEY SMELL)
B	GFP-RFP	All herma	I X F V	Long	More to the salt.(THEY SMELL)
C	WILD	6 males 100 herma	I X F X	Long	They go wherever they want.( THEY DON’T)
D	DAF 18 SILENCED	All herma	I X F X	Less long than the others	They go wherever they want. (THEY DON’T)