CELL MITOSIS

OBJECTIVES

The aim of this practice is to identify the phases of the mitosis in the root of an onion.

MATERIAL 

• Microscope
• Slide and coverslide
• Dropper
• Watch glass.
• Beaker
• Forceps
• Filter paper
• Scissors
• Bunsen burner
• Lighter.
• Needles. 
• Distilled water
• Orcein A
• Orcen B
• Onions
• Water

PROCEDURE

• We keep in a glass the bulb of an onion for a few days, the glass must contain water that is in contact with the roots until the roots measure 3mm.
• Then, we cut the roots and we put them in a watch glass and we add 2mL of Orcein A.
• Heat the watch glass with the orcein until we see gray vapors.
• After that, take the root and put it on the slide and add drops of orcein B. 
• We place the slide on filter paper, wrapping and tighting it.
• We cover it with the coverslide and we observe the results on the microscope. 

RESULTS 

If it has worked out well, the process of mitosis must be observed.

EXTRA

Fossilized lizard